Mineralized Tissue Laboratory

The Mineralized Tissues Laboratory conducts studies on the mechanisms of biologic calcification in health and disease and on the changes that occur in mineralized tissues during development.. Research methodologies include cell culture, x-ray diffraction, FTIR spectroscopy, FTIR micro-spectroscopy, and imaging, TEM and SEM, along with techniques of cell and molecular biology, image analysis, and solution chemistry.

Current investigations are focusing on the role of noncollagenous extracellular matrix proteins in the regulation of bone and tooth mineralization based on solution studies and studies in transgenic animals; the effects of cell and matrix age and composition on in vitro mineral initiation in a model of endochondral ossification, and the changes in the composition of mineral and matrix in osteoporotic and normal bones before and after treatment with anti-resorptive and anabolic agents.

Selected Publications

• Tartaix PH, Doulaverakis M, George A, Fisher LW, Butler WT, Qin C, Salih E, Tan M, Fujimoto Y, Spevak L, Boskey AL. Dentin matrix protein-1: In vitro effects on hydroxyapatite formation provide insights into in vivo functions. J Biol Chem. 2004 Feb 9 [Epub ahead of print]
  
  
• Paschalis EP, Recker R, DiCarlo E, Doty SB, Atti E, Boskey AL. Distribution of collagen cross-links in normal human trabecular bone. J Bone Miner Res. 2003 Nov;18(11):1942-6.
  
  
• Verdelis K, Crenshaw MA, Paschalis EP, Doty S, Atti E, Boskey AL. Spectroscopic Imaging of Mineral Maturation in Bovine Dentin. J Dent Res 82: 697-702, 2003
  
  
• Boskey AL, Moore DJ, Amling M, Canalis E, Delany A: Infrared analysis of the mineral and matrix in bones of osteonectin-null mice and their wildtype controls. JBMR, 18:1005-1011, 2003.
  
  
• Boskey AL, Binderman I, Doty SB: BMP-6 Enhances Mineralization in Differentiating Mesenchymal Cell Cultures. J. Cell Biochem, 84: 509-519, 2002

Laboratory Personnel

Faculty

Steven B Doty, PhD, Senior Member

Philipp Mayer-Kuckuk, Adjunct Instructor

Daniel E. MacDonald, DDS, Adjunct Associate Member

Cathleen L Raggio, MD, Affiliated Clinician

Bruce Rapuano, PhD, Assistant Member

Helen Tudor, Instructor

Pamela Sherman, MD, Affiliated Clinician

Postdoctoral Fellows

Dan Faibish, DDS

Yunfeng Ling, MD

Eric Pourmand, MD

Helen Tudor, PhD

Kostas Verdelis, DDS

Research Associates

Elisa Atti

Lyudmila Spevak

Graduate Students

Rani Roy