Minimally Invasive Anterior Spinal Fusions: Optimization of a Rat Model

Principle Investigator

Matthew Cunningham, MD, PhD

Scope

Using a Lewis rat model, we have previously demonstrated that it is possible to produce anterior spine fusions with delivery of genetically modified stem cells to the intervertebral disk space.  These fusions occurred in over 80% of animals in the most successful treatment group, and fusions were confirmed by manual palpation, static and dynamic x-rays, micro-CT, histology and biomechanics endpoints.  We also observed that fusion at both of the experimental levels was uncommon, that there was a predilection for fusion of the cranial level, and that fusion bone was formed outside of the disk space. The next phase for description of the model is being funded through the North American Spine Society, and is designed to use an identical approach to the model, but with delivery of factors that are expected to further augment bone formation, and to chemically dissolve the intervertebral disk.  We anticipate the increased bone to drive better fusion at the caudal level, and the chemical removal of the disk to allow bone formation within the disk space. The student will be trained to perform (sterile) cell culture techniques, animal (rat) handling techniques, data acquisition, data processing, and data interpretation.  The student will be expected to think like a scientist on both a day-to-day basis for time management and experiment planning, and on a broader “big-picture” basis to resolve problems in the project execution and to play a role in abstract/manuscript preparation.

Contact

Matthew Cunningham MD, PhD
cunninghamm@hss.edu